| Annotated protein: | Sonic hedgehog protein (SHH) (EC 3.1.-.-) (Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains) (ShhNC) [Cleaved into: Sonic hedgehog protein N-product (ShhN) (Shh N-terminal processed signaling domains) (ShhNp)]. Gene symbol: SHH. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63673 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SHH |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus, glutamatergic Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Petralia RS, et al. "Sonic hedgehog distribution within mature hippocampal neurons" Commun Integr Biol. 2011 Nov 1;4(6):775-7 PMID:22446553 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1. immunogold labeling shows Shh at synapses in Ca1, Ca3, and dentate gyrus of the adult rat hippocampus. " Figure 1B-H show typical excitatory synapses based on their prominent postsynaptic density. In these synapses, presynaptic Shh labeling displayed a slightly different pattern from postsynaptic Shh labeling. Presynaptic Shh labeling could be found either directly at the membrane of the synaptic junction (Fig. 1B-D) or on the side of the terminal (Fig. 1H). Postsynaptic Shh labeling, on the other hand, was found mostly on the side, away from the center of the synaptic junction (Fig. 1E-G). Figure 1I-M shows examples of mossy fiber synapses. As for other types of synapses, Shh was found in both the presynaptic mossy terminals and the postsynaptic thorny excrescences. Within the presynaptic mossy terminals, most Shh labeling was clearly seen associated with either dense-cored vesicles (arrows in Figure 1J-M), or other smaller vesicular structures (arrowheads in Figure 1I,J,M). Within the postsynaptic thorny excrescences, Shh was associated with tubulovesicular organelles in some cases (arrowheads in Figure 1I), or positioned quite close to the postsynaptic membrane in other cases (arrowhead in Figure 1L). In addition to synaptic localizations, Shh labeling was found in various tubulovesicular organelles in the soma and dendrites of hippocampal neurons (Fig. 2). Interestingly, some of these Shh-labeled organelles made direct contact with the cell membrane surface, typically near contacts with adjacent processes (Fig. 2A-C). " |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | same authors showed in an earlier publication that SSH localizes to synapses using immuno-EM and confocal fluorescence co-localization with homer/bassoon. @ PMID:30837226 |
| SynGO annotation ID: | 4601 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |