| Annotated protein: | Inward rectifier potassium channel 4 (Inward rectifier K(+) channel Kir2.3) (IRK-3) (Potassium channel, inwardly rectifying subfamily J member 4). Gene symbol: KCNJ4. Taxonomy: Mus musculus (Mouse). Uniprot ID: P52189 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNJ4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | brain, glutamatergic |
| Annotated paper: | Inanobe A, et al. "Inward rectifier K+ channel Kir2.3 is localized at the postsynaptic membrane of excitatory synapses" Am J Physiol Cell Physiol. 2002 Jun;282(6):C1396-403 PMID:11997254 |
| Figure(s): | Figure 2, 3, 4 |
| Annotation description: | Fig. 2. Immunohistochemistry of Kir2.3 (KCNJ4) in mouse brain. - strong immunoreactivity was found in the olfactory bulb (OB), olfactory tubercle (Tu), neocortex (Cx), caudate putamen (CP), and hippocampus (Hip) - weak immunoreactivity was found in the inferior colliculus (IC); no immunoreactivity was found in the cerebellum (Cb) - immunoreactivity was strongly developed in the external plexiform layer (EPL) and weakly developed in the mitral cell layer (MCL) and granule cell layer (GRL) - no immunoreactivity was found in the olfactory nerve layer (ONL) or the glomerular layer (GL) Fig. 3. Subcellular localization of Kir2.3 immunoreactivity in mouse olfactory bulb (OB) shows strong enrichment at the excitatory postsynaptic membrane. Fig4. IP+WB were used to detect PDZ domain-containing proteins coisolated with Kir2.3. PSD-95 (DLG4) and chapsyn-110 (DLG2) were found in the immunocomplex, but SAP102 (DLG3), SAP97(DLG1), S-SCAM (Magi2), and ZO-1 (TJP1) were not. based on the protein's transmembrane topology, "integral component of ..." was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4599 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |