| Annotated protein: | Metabotropic glycine receptor (mGlyR) (G-protein coupled receptor 158). Gene symbol: GPR158. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8C419 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GPR158 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Annotated paper: | Condomitti G, et al. "An Input-Specific Orphan Receptor GPR158-HSPG Interaction Organizes Hippocampal Mossy Fiber-CA3 Synapses" Neuron. 2018 Oct 10;100(1):201-215.e9 PMID:30290982 |
| Figure(s): | 2G, 2H, 2I |
| Annotation description: | Figure 2G: Subcellular fractionation followed by Western Blot analysis show that GPR158 is strongly enriched in the Triton-insoluble fraction containing PSD95, suggesting a postsynaptic localization. Figure 2H: In utero electroporation of embryonic day E15.5 mouse embryos was used to express a GFP reporter and HA-tagged GPR158 (GFP-T2A-HA-GPR158) in CA3 pyramidal neurons and imaged sparsely transduced cells using structured illumination microscopy (SIM) at P21. HA-GPR158 displayed a specific, punctate distribution that was restricted to the proximal portion of GFP-labeled CA3 apical dendrites. Figure 2I: HA-GPR158 puncta localize to the heads of thorny excrescences. Not all spines display detectable HA immunofluorescence. Taken together, the results show that GPR158 is enriched in the PSD. Based on the known transmembrane topology of this protein (e.g. PMID: 34793198), "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution Western blot Biochemical fractionation (generic) |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4592 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |