| Annotated protein: | Potassium channel tetramerization domain containing 12. Gene symbol: KCTD12. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I5Y7I2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCTD12 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic membrane (GO:0042734) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Schwenk J, et al. "Native GABA(B) receptors are heteromultimers with a family of auxiliary subunits" Nature. 2010 May 13;465(7295):231-5 PMID:20400944 |
| Figure(s): | Figure 2b, 2c |
| Annotation description: | Subcellular distribution of KCTD12 was assessed in pre-embedding immunoelectron microscopy (Figure 2b) and in immunolabelling of freeze-fracture replicas of the hippocampal CA1 region (Figure 2c). Both techniques localized the anti-KCTD12 immunoreactivity to the plasma membrane of presynaptic and postsynaptic compartments of principal cells that were also stained by anti-GABAB1. Immunogold particles were most abundant at the extrasynaptic plasma membrane of dendritic spines and dendritic shafts of pyramidal cells, but also found at the presynaptic membranes of boutons of putative excitatory cells. No antibody specificity control in manuscript (minus primary or pre-absorption control) |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4589 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |