Annotated protein:Potassium channel tetramerization domain containing 16. Gene symbol: KCTD16. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6A6D0
antibody wiki:
SynGO gene info:SynGO data @ KCTD16
Ontology domain:Cellular Component
SynGO term:presynaptic membrane (GO:0042734)
Synapse type(s):hippocampus
Annotated paper:Schwenk J, et al. "Native GABA(B) receptors are heteromultimers with a family of auxiliary subunits" Nature. 2010 May 13;465(7295):231-5 PMID:20400944
Figure(s):Figure 2b, 2c
Annotation description:Subcellular distribution of KCTD16 was assessed in pre-embedding immunoelectron microscopy (Figure 2b) and in immunolabelling of freeze-fracture replicas of the hippocampal CA1 region (Figure 2c). Both techniques localized the anti-KCTD16 immunoreactivity to the plasma membrane of presynaptic and postsynaptic compartments of principal cells that were also stained by anti-GABAB1. Immunogold particles were most abundant at the extrasynaptic plasma membrane of dendritic spines and dendritic shafts of pyramidal cells, but also found at the presynaptic membranes of boutons of putative excitatory cells.

No antibody specificity control in manuscript (minus primary or pre-absorption control)
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Annotator(s):Robin Clement
Guus Smit
Matthijs Verhage
ORCID:0000-0002-2286-1587
0000-0002-2514-0216
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:4588
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology