| Annotated protein: | Protein arginine N-methyltransferase 8 (EC 2.1.1.319) (Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4). Gene symbol: PRMT8. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6PAK3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRMT8 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic actin cytoskeleton (GO:1905274) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Lo LH, et al. "The Protein Arginine Methyltransferase PRMT8 and Substrate G3BP1 Control Rac1-PAK1 Signaling and Actin Cytoskeleton for Dendritic Spine Maturation" Cell Rep. 2020 Jun 9;31(10):107744 PMID:32521269 |
| Figure(s): | Figure 1G, 2, 4B-D 5, 6 |
| Annotation description: | - Arginine methylation is a major PTM that regulates chromatin structure, transcription, and splicing within the nucleus. - Figure 1G: Western blot shows upregulated protein arginine methyltransferase PRMT8 expression in the hippocampus when dendritic spine maturation occurs. - Figure 2: Depletion of PRMT8 by shRNA knockdown leads to overabundance of filopodia and mislocalization of excitatory synapses. - Figure 4B-D: spine morphology alterations can also be found in hippocampi of PTM8 KO mice. - Figure 5-6 : Spinning disc, confocal microscopy, FRAP assays and Western blots show that PRMT8 promotes dendritic spine morphology through methylation of the dendritic RNA-binding protein G3BP1 and suppression of the Rac1-PAK1 signaling pathway to control synaptic actin dynamics. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Fluorescence recovery after photobleaching (FRAP) Western blot IP + WB/MSMS |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4586 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |