| Annotated protein: | Protein arginine methyltransferase 8. Gene symbol: PRMT8. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1LWG2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRMT8 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lo LH, et al. "The Protein Arginine Methyltransferase PRMT8 and Substrate G3BP1 Control Rac1-PAK1 Signaling and Actin Cytoskeleton for Dendritic Spine Maturation" Cell Rep. 2020 Jun 9;31(10):107744 PMID:32521269 |
| Figure(s): | Figure 1A-D |
| Annotation description: | - PRMT is a family of enzymes that catalyze the addition of the methyl group to arginine residue of the substrate. - PRMT8 is required for synaptic plasticity in the hippocampus and memory formation. - Expression of PRMT8 is is largely restricted in the brain and is the only membrane-bound PRMT via N-terminal myristoylation. - Figure 1A: In situ hybridization of rat hippocampal neurons. Prmt8 mRNA puncta were found in dendrites. - Figure 1B: Western blot shows that PRMT8 protein is present in the synapse-enriched synaptoneurosome fraction from the forebrain - Figure 1C: Immunofluorescence staining of PRMT8 protein and PSD-95 in primary hippocampal neurons showed that PRMT8 exists as distinct puncta on dendrites and some PRMT8 puncta were partially overlapped with the postsynaptic protein PSD-95. - Figure 1D: Hippocampal neurons transfected with GFP-tagged PRMT8 or GFP, followed by staining with GFP antibody at 19 DIV. PRMT8 appeared as discrete puncta in the dendrite, and some were localized in the dendritic spine heads. -Figure 4A: Western blot in PRMT8 +/+ , +/- and -/- mice shows antibody specificity. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4585 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |