| Annotated protein: | BTB/POZ domain-containing protein KCTD12 (Pfetin) (Predominantly fetal expressed T1 domain). Gene symbol: KCTD12. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6WVG3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCTD12 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone membrane (GO:0048787) |
| Annotated paper: | Bhandari P, et al. "GABA(B) receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals" Elife. 2021 Apr 29;10:e68274 PMID:33913808 |
| Figure(s): | Figure 3d, Figure 3 supplement 2 |
| Annotation description: | - K+-channel tetramerization domain-containing proteins (KCTDs) are auxiliary GABAB receptor (GBR) subunits. - Experiments were performed at the synaptic connection from medial habenula (MHb) to interpeduncular nucleus (IPN). -Using pre-embedding immunogold labeling, imaged with TEM, KCTD12 showed "particles for KCTD12 were found mostly on the postsynaptic side of asymmetrical synapses and only few synapses exhibited presynaptic KCTD12 labeling along synaptic and extrasynaptic membrane (Figure 3D, Figure 3 - figure supplement 2)" - Figure 2B: immunolabeling of WT and KCTD12 KO mice shows antibody specificity |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4581 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |