| Annotated protein: | Disco-interacting protein 2 homolog A (DIP2 homolog A) (EC 6.2.1.1). Gene symbol: DIP2A. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BWT5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DIP2A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic actin cytoskeleton (GO:1905274) |
| Annotated paper: | Ma J, et al. "Autism candidate gene DIP2A regulates spine morphogenesis via acetylation of cortactin" PLoS Biol. 2019 Oct 10;17(10):e3000461 PMID:31600191 |
| Figure(s): | Figure 1, 2, 3 |
| Annotation description: | - DIP2A is involved in acetylated coenzyme A (Ac-CoA) synthesis and is primarily expressed in the brain regions with abundant pyramidal neurons. - DIP2A is localized to dendritic spines in excitatory neurons (Fig.1) - shown with Western Blot of synaptosomes and fluorescence microscopy of cultured neurons - deletion of Dip2a in mice induces defects in spine morphogenesis along with thin postsynaptic density (PSD), and reduced synaptic transmission of pyramidal neurons (Fig. 2) - as shown with fluorescence microscopy, whole-cell patch clamp, Western Blot and TEM - DIP2A interacts with cortactin, an activity-dependent spine remodelling protein, and modulates its acetylation (Fig. 3) - shown by Western Blot of mural brain lysates and fluorescence microscopy - Acetylation cortactin is critical for DIP2A-regulated synaptic transmission. Acetylation mimetic cortactin rescues synaptic defects. (Fig. 6) - shown with whole-cell patch clamp of 8KQ infected Dip2a KO neurons at DIV10 |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Western blot Biochemical fractionation (generic) Whole-cell patch clamp |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4576 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |