| Annotated protein: | Tyrosine-protein phosphatase non-receptor type 1 (EC 3.1.3.48) (Protein-tyrosine phosphatase 1B) (PTP-1B). Gene symbol: PTPN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P20417 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PTPN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Fuentes F, et al. "Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning" PLoS One. 2012;7(7):e41536 PMID:22844492 |
| Figure(s): | Fig. 1, 2 |
| Annotation description: | Figure 1 shows the colocalization of PTP1B (PTPN1) and synapsin-1 at synaptic puncta of cultured rat hippocampal neurons using confocal microscopy. Authors observe: "PTP1B displays a punctate distribution in dendritic shafts and in thin axons. Occasionally, PTP1B puncta locates in dendritic protrusions. In neurons of DIV21, PTP1B puncta sometimes can be seen at the heads of mushroom-like spines". Figure 2 shows localization and dynamics of GFP-PTP1B in dendritic protrusions of cultured rat hippocampal neurons. Authors observe: "GFP-PTP1B is observed in mushroom-like dendritic spines as fingerlike extensions from dendritic shafts (Figure 2C). Quantitative analysis reveals that GFP-PTP1B extensions are present in 11.4% of the dendritic protrusions at DIV10 and in 13.5% at DIV21 (Table 2). These extensions can be found protruding towards PSD-95 clusters detected by immunofluorescence (Figure 2F)." The scarce colocalization with synaptic markers and the low frequency of localization in dendritic spines suggest that PTP1B may be a transient component of this compartment. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4557 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |