| Annotated protein: | CD44 antigen (Extracellular matrix receptor III) (ECMR-III) (GP90 lymphocyte homing/adhesion receptor) (HUTCH-I) (Hermes antigen) (Hyaluronate receptor) (Phagocytic glycoprotein 1) (PGP-1) (Phagocytic glycoprotein I) (PGP-I) (CD antigen CD44). Gene symbol: CD44. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P26051 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CD44 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Roszkowska M, et al. "CD44: a novel synaptic cell adhesion molecule regulating structural and functional plasticity of dendritic spines" Mol Biol Cell. 2016 Dec 15;27(25):4055-4066 PMID:27798233 |
| Figure(s): | Fig. 1B |
| Annotation description: | Immunogold particles were frequently associated with asymmetric synapses (Figure 1B). Of interest, CD44 labeling in the CA3 stratum radiatum was detected at both presynaptic and postsynaptic sites. The quantitative analysis of the number of gold particles in dendritic spines and axonal boutons confirmed a significant association between gold particles and these compartments compared with an expected random distribution (dendritic spines: x^2 = 1140.37, p < 0.00001; axonal boutons: x^2 = 217.739, p < 0.00001). Specificity of the antibody (polyclonal sheep IgG; R&D Systems) has been tested in PMID 25300795 (Skupien et al., 2014, JCS 127:5038; Fig. 2) by shRNA knock down. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | This paper contributes to the determination of the antibody specificity and contributes immunogold localization at postnatal day 10. @ PMID:25300795 |
| SynGO annotation ID: | 4547 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |