| Annotated protein: | Potassium voltage-gated channel subfamily C member 3 (KSHIIID) (Voltage-gated potassium channel subunit Kv3.3). Gene symbol: KCNC3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q63959 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNC3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle endocytosis (GO:1900242) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Wu XS, et al. "Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles" Neuron. 2021 Mar 17;109(6):938-946.e5 PMID:33508244 |
| Figure(s): | Figure 1 and 2 |
| Annotation description: | Figure 1: Whole-cell voltage-clamped recordings with a 20-ms depolarization from -80 mV to +10 mV (Depol20ms) was applied to induce slow endocytosis (time constant [τ] > ~10 s). The Depol20ms protocol induced a calcium current (ICa) and a membrane capacitance jump (ΔCm) followed by a mono-exponential decay and an initial decay rate. Compared with WT calyces, the Ratedecay, but not ΔCm or ICa charge (QICa), was reduced significantly in Kv3.3-/- calyces, and the Cm decay was much prolonged. Similar results were observed in homozygous Kv3.3G592R KI mice (called Kv3.3G592R mice). This Kv3.3G592R mutant was generated such that the phenotype shows normal Kv3.3 currents but fail to trigger actin nucleation. Figure 2: The Depol20ms protocol at 10Hz frequency induced rapid endocytosis. Compared with the WT, the Ratedecay, but not QICa, in Kv3.3-/- or Kv3.3G592R calyces decreased significantly; the Cm decay was much prolonged in Kv3.3-/- or Kv3.3G592R calyces. These results suggest that the Kv3.3 channel is involved in rapid endocytosis. Overall, KV3.3 KO causes a significant delay in both slow and rapid endocytosis in the calyx of held synapses. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4545 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |