| Annotated protein: | Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Brain calcium channel I) (BI) (Calcium channel, L type, alpha-1 polypeptide isoform 4) (Voltage-gated calcium channel subunit alpha Cav2.1). Gene symbol: CACNA1A. Taxonomy: Oryctolagus cuniculus (Rabbit). Uniprot ID: P27884 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1A |
| Ontology domain: | Biological Process |
| SynGO term: | voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels (GO:0099626) |
| Annotated paper: | Berkefeld H, et al. "BKCa-Cav channel complexes mediate rapid and localized Ca2+-activated K+ signaling" Science. 2006 Oct 27;314(5799):615-20 PMID:17068255 |
| Figure(s): | Figure 1-4 |
| Annotation description: | Figure S2B: Co-purification of BKCa and Cav channels from Xenopus oocytes expressing BKCa (BKα, BKβ4) and Cav2.1 (Cav2.1(I1520H), Cavβ1b or Cavβ3, α2δ) channels. Figure 3A: Electrophysiological recordings show that BKCa and Cav2.1 channels functionally are coupled. Figure 3B: The coupling between both channels were mandatory for the activation of BKCa in the physiological voltage range as shown by control experiments lacking expression of the Cav channels. The amplitude of the Cav channel-activated BKCa current was strongly voltage-dependent and exhibited a bell-shaped steady-state current-voltage (I-V) relation with a peak amplitude at about 20 mV. In particular, an increase in open probability and faster activation kinetics of the Cav channels promote accelerated coupling (Fig. 3A) and an increase in the BKCa currents (Fig. 3B), whereas the decrease in Ca2+ current amplitude (due to a reduced driving force at voltages approaching the Ca2+ reversal potential) leads to reduction and cessation of BKCa currents at voltages >20 mV (Fig. 3B). Figure 6: BKCa currents were eliminated by application of w-agatoxin IVA, a specific blocker of Cav2.1 further confirming the functional coupling between BKCa and Cav2.1. 26/03/2021 Dnyanada: The term "voltage-gated calcium channel activity.." was chosen as Cav2.1 (CACNA1A) contributes to an influx of Ca2+ (also represented in Figure 3A). As this Cav2.1 functional couple with the BKCa channel (which are localized in the presynaptic terminal; SynGO ID 1446), it is assumed the effect regulates the presynaptic cytosolic calcium levels. |
| Evidence tracking, Biological System: | Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4537 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |