| Annotated protein: | Ubiquitin carboxyl-terminal hydrolase CYLD (EC 3.4.19.12) (Deubiquitinating enzyme CYLD) (Ubiquitin thioesterase CYLD) (Ubiquitin-specific-processing protease CYLD). Gene symbol: CYLD. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q66H62 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CYLD |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Thein S, et al. "CaMKII mediates recruitment and activation of the deubiquitinase CYLD at the postsynaptic density" PLoS One. 2014 Mar 10;9(3):e91312 PMID:24614225 |
| Figure(s): | Fig. 1Aa,b |
| Annotation description: | Fig. 1 A shows by immunogold EM localization that the CYLD protein is localized in the postsynaptic density. Immunoreactivity increases after 2 min of NMDA treatment. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | Fig. 3A shows the immunogold EM localization of phosphorylated CYLD in the postsynaptic density of excitatory synapses in hippocampal primary cultured neurons. Labeling is strongly enhanced by 2-min treatment with NMDA. @ PMID:24928390 Fig. 2B shows the enrichment of CYLD with the postsynaptic density protein fraction of rat forebrain in a western blot. The same antiserum was used in Fig. 1 A in Thein et al. (2014). @ PMID:23146630 |
| SynGO annotation ID: | 4534 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |