| Annotated protein: | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (EC 2.4.1.255) (O-GlcNAc transferase subunit p110) (O-linked N-acetylglucosamine transferase 110 kDa subunit) (OGT). Gene symbol: OGT. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CGY8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ OGT |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse assembly (GO:0051963) |
| Synapse type(s): | hippocampus, glutamatergic cerebral cortex, glutamatergic |
| Annotated paper: | Lagerlof O, et al. "O-GlcNAc transferase regulates excitatory synapse maturity" Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1684-1689 PMID:28143929 |
| Figure(s): | Figs. 2-5 |
| Annotation description: | Fig. 4 indicates that OGT regulates the number but not the size of mature synaptic contacts as determined by counting number and puncta size of pre- (VGLUT) and postsynaptic (PSD-95) markers. Fig. 5 shows that there are fewer and more immature spines of OGT KO neurons as assessed by the analysis of spines of GFP-expressing neurons. 15/02/2021 Dnyanada: The paper demonstrates a reduction in the number of mature synapses as well as in the morphologically mature spines in OGT KO neurons. Figure 2 and 3 also demonstrate a reduction in the surface expression of GluR2 and GluR3 AMPAR subunits in OGT KO neurons. As the paper does not causally (and chronologically) link these two effects, a separate annotation for the term "regulation of neurotransmitter receptor localisation to postsynaptic specialisation membrane" was made (SynGO ID: 4521). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4525 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |