| Annotated protein: | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (EC 2.4.1.255) (O-GlcNAc transferase subunit p110) (O-linked N-acetylglucosamine transferase 110 kDa subunit) (OGT). Gene symbol: OGT. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P56558 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ OGT |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | cerebellum, GABAergic cerebellum, glutamatergic Cerebellar granule cell to Purkinje cell synapse |
| Annotated paper: | Akimoto Y, et al. "Localization of the O-GlcNAc transferase and O-GlcNAc-modified proteins in rat cerebellar cortex" Brain Res. 2003 Mar 21;966(2):194-205 PMID:12618343 |
| Figure(s): | Fig. 5C,D, Fig. 6A |
| Annotation description: | Fig. 5 C shows the synaptic region where a basket cell axon contacts the soma of a Purkinje cell. Some gold particles are seen in the presynaptic terminal of a climbing fiber. Fig. 5 D shows synapses in the molecular layer. Many particles are seen in the presynaptic terminals. Particles are also seen in the postsynaptic terminal. Fig. 6 A provides a quantitative analysis of the subcellular distribution of OGT with labelling of presynaptic and postsynaptic terminals. Fig. 2 A shows an immunoblot of cerebellar lysate with anti-OGT antibody labelling a single band of 110 kDa. 12/02/2021 Dnyanada: The protein is also expressed in the postsynaptic cytosol (linked SynGO ID: 4524). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4522 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |