| Annotated protein: | UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (EC 2.4.1.255) (O-GlcNAc transferase subunit p110) (O-linked N-acetylglucosamine transferase 110 kDa subunit) (OGT). Gene symbol: OGT. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CGY8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ OGT |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lagerlof O, et al. "O-GlcNAc transferase regulates excitatory synapse maturity" Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1684-1689 PMID:28143929 |
| Figure(s): | Fig. 1 |
| Annotation description: | OGT is enriched in the postsynaptic density fraction of excitatory synapses as shown by cell fractionation and immunoblot analysis (Fig 1A,B) and by colocalization with the postsynaptic marker protein PSD-95 (Fig. 1C). From the resolution of light microscopy, one cannot discriminate between pre-and postsynaptic localization. Another group (Akimoto et al. (2003) PMID: 12618343) has shown in the cerebellum that OGT is pre-and postsynaptic. An immunoblot of the antibody is shown in Fig. 1A. 12/02/2021 Dnyanada: Due to low resolution and lack of a presynaptic marker in the staining (to negate presynaptic localisation, Figure 1C), the term "synapse" was chosen. However, one must account for the enrichment of OGT in PSD fractionation as well as its crucial role in regulating postsynaptic AMPAR expression (SynGO ID: 4521) in favour of its postsynaptic localisation. |
| Evidence tracking, Biological System: | Cultured neurons Cell-free system |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | This paper shows by cell fractionation and immunoblot analysis that OGT is enriched in synaptic nerve terminals (synaptosomes). @ PMID:19004831 |
| SynGO annotation ID: | 4520 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |