| Annotated protein: | Neurocan core protein (Chondroitin sulfate proteoglycan 3). Gene symbol: NCAN. Taxonomy: Mus musculus (Mouse). Uniprot ID: P55066 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NCAN |
| Ontology domain: | Cellular Component |
| SynGO term: | perisynaptic extracellular matrix (GO:0098966) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Sullivan CS, et al. "Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons" Sci Rep. 2018 Apr 18;8(1):6143 PMID:29670169 |
| Figure(s): | Fig. 2 E,F |
| Annotation description: | Neurocan localization in the prefrontal cortex (P18) was probed at the electron microscope level by immunogold labeling. Neurocan labeling was sometimes seen at the somal membrane in proximity to synaptic vesicle-containing axon terminals Fig. 2E shows an electron micrograph of immunogold labeling for neurocan along the membrane of a neuronal soma (black arrows point to examples). Fig. 2F depicts an electron micrograph of an inhibitory axon terminal onto a neuronal soma. Fig. 2G verifies the specificity of the antibody. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4518 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |