| Annotated protein: | Neurocan core protein (Chondroitin sulfate proteoglycan 3). Gene symbol: NCAN. Taxonomy: Mus musculus (Mouse). Uniprot ID: P55066 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NCAN |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Mohan V, et al. "Neurocan Inhibits Semaphorin 3F Induced Dendritic Spine Remodeling Through NrCAM in Cortical Neurons" Front Cell Neurosci. 2018 Oct 9;12:346 PMID:30356641 |
| Figure(s): | Fig. 3 A-C |
| Annotation description: | Neurocan inhibits Sema3F-induced spine retraction. Fig. 3 A shows apical dendrites and spines of mouse cortical neurons expressing EGFP in cultures treated with Fc or Sema3F-Fc. Pretreatment with 20 nM Neurocan prevented Sema3F-Fc induced spine collapse but did not affect spine density in Fc-treated control cultures. (B) Quantification of experiment in panel A, showing significant reduction in mean spine density upon Sema3F-Fc treatment by Neurocan. (C) NrCAM enhances Neurocan action as lower concentrations of Neurocan (8 nM) have a similar effect in preventing Sema3F-Fc induced spine collapse and did not affect spine density in Fc-treated control cultures. Related to annotations ID 1889 and ID 2762 |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4517 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |