| Annotated protein: | Voltage-dependent N-type calcium channel subunit alpha-1B (Brain calcium channel III) (BIII) (Calcium channel, L type, alpha-1 polypeptide isoform 5) (Voltage-gated calcium channel subunit alpha Cav2.2). Gene symbol: CACNA1B. Taxonomy: Mus musculus (Mouse). Uniprot ID: O55017 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1B |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone (GO:0048786) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Lenkey N, et al. "Tonic endocannabinoid-mediated modulation of GABA release is independent of the CB1 content of axon terminals" Nat Commun. 2015 Apr 20;6:6557 PMID:25891347 |
| Figure(s): | Figure 3, S3 |
| Annotation description: | Figure 3a-g: SDS-digested replica immunogold labelling demonstrates that the Cav2.2 subunit of the voltage-gated Ca2+ channels is localized to the presynaptic active zone of CCK expressing GABAergic interneurons of the hippocampus. Figure 3i: The Cav2.2 subunit immunolabelling was abolished in Cav2.2-/- mice demonstrating the specificity of the antibody. S3c-d: Co-labelling of axon terminal with CB1 (10 nm gold) and Cav2.2 subunit (15 nm gold) further shows localisation of Cav2.2 immunogold particles in the active zone (inset: putative AZs are coloured in orange). 12/01/2021 Dnyanada: The paper uses active zone marker RIM1/2 labelling to mark the active zone. They justify not using colocalisation experiments as the primary antibodies used for both Cav2.2 and the active zone marker RIM1/2 are raised in the same species (rabbits). The annotation is linked to the Uniprot accession ID for mouse, however, it should be noted that both rat and mouse were used for the EM data. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Using the same approach and Ab, Eltes et al. (2017) demonstrated that the Cav2.2 subunit is also confined to the active zone of hippocampal glutamatergic terminals, establishing synapses on two distinct interneurons: parvalbumin- and mGluR1a-expressing interneurons @ PMID:28115484 |
| SynGO annotation ID: | 4512 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |