| Annotated protein: | Ephrin type-A receptor 7 (EC 2.7.10.1) (EPH homology kinase 3) (EHK-3). Gene symbol: EPHA7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P54759 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPHA7 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Lai KO, et al. "Expression of Eph receptors in skeletal muscle and their localization at the neuromuscular junction" Mol Cell Neurosci. 2001 Jun;17(6):1034-47 PMID:11414792 |
| Figure(s): | Fig. 3, Fig. 4 |
| Annotation description: | Fig. 3: colocalization of EPHA7 with AChR at the NMJ. Fig. 4: colocalization of EPHA7 with postsynaptic AChR but not with presynatic SV2 at the NMJ. 25/4/2017 Pim - The authors discuss distinguising presynaptic, postsynaptic and Swann cell localization of EPHA7: "It is possible that the receptors localized at NMJ are present on the presynaptic terminal of motor neurons rather than the postsynaptic apparatus on muscle fiber. To confirm the concentration of EphA4, EphA7, and ephrin-A2 on the postsynaptic specializations, immunostaining was performed on denervated muscle. Upon denervation, the axon terminal normally degenerates by day 4 after nerve cut. However, staining by antibody against EphA4, EphA7, or ephrin-A2 remained at the NMJ twenty days after denervation (Figs. 4A-4C). In contrast, antibody against the presynaptic protein synapsin I stained intensely at the adult NMJ but the staining disappeared at day 4 after denervation (Fig. 4D). Although both the ligands and receptors remained at the NMJ after nerve injury, the intensity of staining at the synapses reduced considerably. This expression profile is similar to that observed for the localization of some other postsynaptic proteins, such as the muscle specific kinase (MuSK) and neuregulin (NRG), in denervated muscle (Bowen et al., 1998; Rimer et al., 1998)." ... "To further confirm the concentration of Eph receptors on the postsynaptic side of NMJ, the localization of EphA7 was compared with that of SV2, which is concentrated in the presynaptic terminals of motor neurons. Much of the EphA7 staining did not overlap with the SV2 staining, indicating that the localization of EphA7 was distinct from that of SV2 (Fig. 4E). Taken together, our data strongly suggests that there is localization of EphA4, EphA7, and ephrin-A2 at the postsynaptic apparatus." - Eph7A has a TM domain. Authors conclude "Importantly, both receptors, as well as the ligand ephrin-A2, are concentrated at the postsynaptic membrane on muscle fiber." we could make a "integral component of postsynaptic membrane" for this. 17/5/2017 Pim - The postsynaptic nAChRs are a marker for the "postsynaptic specialization membrane". Therefore this term was selected over "postsynaptic membrane" localization of EPHA7. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 449 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |