| Annotated protein: | cAMP-dependent protein kinase type I-beta regulatory subunit. Gene symbol: PRKAR1B. Taxonomy: Mus musculus (Mouse). Uniprot ID: P12849 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRKAR1B |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | forebrain, glutamatergic |
| Annotated paper: | Biesemann C, et al. "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting" EMBO J. 2014 Jan 13;33(2):157-70 PMID:24413018 |
| Figure(s): | Supplemental Table 2 |
| Annotation description: | The authors used synaptosomes derived from vGlut-Venus knockin mice to enrich for glutamatergic presynaptic terminals. Mass spectrometry was performed to detect protein content, which identified PKA RI beta. Although this method favors the detection of presynaptic proteins, postsynaptic or extrasynaptic localization of RI beta cannot be excluded with this method. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | none of the above |
| Evidence tracking, Experiment Assay: | Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 443 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |