| Annotated protein: | SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2). Gene symbol: SHANK3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JLU4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SHANK3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Petralia RS, et al. "Ontogeny of postsynaptic density proteins at glutamatergic synapses" Mol Cell Neurosci. 2005 Jul;29(3):436-52 PMID:15894489 |
| Figure(s): | Fig 1, fig 2, fig 9, table 1. |
| Annotation description: | Authors use an antibody which revealed multiple bands, as expected from the recognition of different Shank variants. Authors described that all prominent bands increased with age in hippocampal preparations as assessed by WB, and a band at 240kDa was very prominent in PSDII fractions prepared from forebrain, which they clarify is the final PSD isolate. Immunogold EM revealed that Shank3 is present in synapses at all ages (P2, P10, P35) reaching higher levels in adulthood. The immunogold labeling formed clusters within the PSD and also adjacent to it in all studied ages. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| Additional literature: | Literal: An antibody generated against a C-terminal region of Shank3 (aa 1379-1675) was used to examine the ultrastructural distribution of the Shank proteins in brain. This antibody recognizes multiple bands on brain immunoblots, including major bands of approximately 160- 180 kDa and approximately 210 kDa in forebrain and cerebellum (Figure 6A), similar to those seen with other Shank antibodies (see Naisbitt et al., 1999). The different size bands presumably derive from the multiple Shank genes and splice variants. All Shank immunoreactivity was blocked by incubation of the Shank Ab with the Shank fusion protein antigen (Figure 6A). @ PMID:10433269 |
| SynGO annotation ID: | 425 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |