| Annotated protein: | Neurobeachin. Gene symbol: NBEA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6AP41 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NBEA |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic membrane (GO:0098890) |
| Synapse type(s): | cerebellum, GABAergic Cerebellar Golgi cell to granule cell synapse |
| Annotated paper: | Wang X, et al. "Neurobeachin: A protein kinase A-anchoring, beige/Chediak-higashi protein homolog implicated in neuronal membrane traffic" J Neurosci. 2000 Dec 1;20(23):8551-65 PMID:11102458 |
| Figure(s): | Fig. 8C |
| Annotation description: | Neurobeachin is a peripheral membrane-associated protein. It is found in a subpopulation of synapses, where it is concentrated at the postsynaptic plasma membrane. Fig. 8C shows an example of the association with the postsynaptic membrane of a GABAergic synapse. 19/01/2021 Dnyanada: Author's attempt at verifying the specificity of the anti-Neurobeachin antibody. "To determine the tissue specificity of neurobeachin at the protein level, a Western blot of homogenates from various mouse tissues was probed with an affinity-purified antibody that had been raised against recombinant neurobeachin region B (Fig. 5B). A strong protein band of molecular size far above the 206 kDa standard was detectable only in brain lysates, with similar intensities in forebrain, cerebellum, and brainstem; a very faint band was seen in stomach. This band could be specifically immunoprecipitated from brain lysate, and its labelling is blocked by preincubation of the antibody with neurobeachin region B but not by BGL region B (data not shown)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| SynGO annotation ID: | 4206 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |