| Annotated protein: | Receptor-type tyrosine-protein phosphatase delta (Protein-tyrosine phosphatase delta) (R-PTP-delta) (EC 3.1.3.48). Gene symbol: PTPRD. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q64487 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PTPRD |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Park H, et al. "Splice-dependent trans-synaptic PTPdelta-IL1RAPL1 interaction regulates synapse formation and non-REM sleep" EMBO J. 2020 Jun 2;39(11):e104150 PMID:32347567 |
| Figure(s): | Figure 1F-I |
| Annotation description: | Figure 1 PTPδ-tdTomato protein mainly localizes to presynaptic sites at excitatory, but not inhibitory, synapses in the mouse hippocampus (F-I) Ultrastructural localization of PTPδ-tdTomato protein (red arrows; DAB staining) at axon terminals juxtaposed to electron-dense postsynaptic densities (purple arrows; PSDs) and also at vGlut2-positive excitatory synaptic axon terminals (green arrows; immunogold staining) but not at GAD67/65-positive inhibitory synaptic axon terminals (blue arrows; immunogold staining) in the stratum lacunosum-molecular (SLM) region of the CA1 hippocampal region (P21). 19/01/2021 Dnyanada: Figure EV1J: PTPδ and PTPδ-tdTomato proteins distribute similarly to subcellular brain fractions, as shown by immunoblot analysis of subcellular fractions from the whole brain of PTPδ-tdTomato reporter mice (P21). Analysis of immunoblots reveals no difference in the subcellular localization of PTPδ-tdTomato fusion proteins as compared to wild-type PTPδ indicating that insertion of tdTomato does not alter synaptic localisation of PTPδ. PTPRD_MOUSE (Q64487) demonstrates transmembrane topology of the protein, in favour of the term "integral component..." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hwajin Jung (ORCID:0000-0001-6194-9648) Eunjoon Kim (ORCID:0000-0001-5518-6584) |
| Lab: | Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS) and Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea |
| SynGO annotation ID: | 4204 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |