| Annotated protein: | Cyclin-dependent kinase-like 5. Gene symbol: CDKL5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZG85 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CDKL5 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Zhu YC, et al. "Palmitoylation-dependent CDKL5-PSD-95 interaction regulates synaptic targeting of CDKL5 and dendritic spine development" Proc Natl Acad Sci U S A. 2013 May 28;110(22):9118-23 PMID:23671101 |
| Figure(s): | Fig. 1, 3, S1, S2 |
| Annotation description: | Fig. 1 colocalization of CDKL5 with PSD-95, coimmunoprecipitation of CDKL5 with PSD-95 but not with Synaptophysin. Fig. 3 localization of CDKL5 to synapses depend on PSD-95 Fig. S2 CDKL5 is enriched in the PSD fraction 14/4/2017 Pim Fig.S1 (binding of recombinant PSD-95 and CDKL5) shows that the interaction of these proteins is direct. This places CDKL5 in the same cellular compartment as PSD-95 --> postsynapse. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Cell-free system |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) IP + WB/MSMS Protein-protein interaction (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 420 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |