| Annotated protein: | Interleukin-1 receptor accessory protein (IL-1 receptor accessory protein) (IL-1RAcP) (EC 3.2.2.6) (Interleukin-33 receptot beta chain). Gene symbol: IL1RAP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61730 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IL1RAP |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | Yoshida T, et al. "Interleukin-1 receptor accessory protein organizes neuronal synaptogenesis as a cell adhesion molecule" J Neurosci. 2012 Feb 22;32(8):2588-600 PMID:22357843 |
| Figure(s): | Fig.4 A |
| Annotation description: | Figure 4. Effects of IL-1RAcP isoforms on synapse formation. A, Effects of FLAG-IL-1RAcP and FLAG-IL-1RAcPb on the dendritic protrusions of cultured cortical neurons and the accumulation of Bassoon signals along dendrites. The figure shows the staining with ani-FLAG antibodiees of neurons trasnfected with FLAG-IL-1RAcP and FLAG-IL-1RAcPb cDNAs. Literal: "We then examined the effects of expression of IL-1RAcP and IL-1RAcPb in cultured cortical neurons on synapse formation. Transfection of cultured cortical neurons at DIV 12 with the expression vector for FLAG-IL-1RAcP increased punctate immunostaining signals for Bassoon but exerted little effect on dendritic protrusions (Fig. 4). On the other hand, transfection of FLAG-IL-1RAcPb stimulated both the accumulation of Bassoon signals along the dendrites and formation of dendritic protrusions (Fig. 4). These results suggest that both IL-1RAcP and IL-1RAcPb have the ability to induce presynaptic differentiation, while the spinogenic activity is specific for IL-1RAcPb." 28/01/2021 Dnyanada: The paper demonstrates localisation of FLAG-IL-1RAcP in dendritic protrusions, and does not provide any "synaptic resolution". It also shows that IL-1RAcP plays a role in synapse formation. Based on this, the term "synapse" was chosen. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 4187 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |