| Annotated protein: | PTPRF interacting protein alpha 2. Gene symbol: PPFIA2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M8A4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization (GO:0099572) |
| Annotated paper: | Wyszynski M, et al. "Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA receptor targeting" Neuron. 2002 Mar 28;34(1):39-52 PMID:11931740 |
| Figure(s): | Fig. 5, Fig. 3 |
| Annotation description: | - Postembedding immunogold EM of adult rat cerebral cortex was performed using 254.2 anti-liprin2-antibody. - Fig5B-C: EM images show the expression of gold particles adjacent to the postsynaptic membrane. - Fig5E quantifies the distribution of gold particles relative to the postsynaptic membrane (value of 0). The high peak within 50 nm of the postsynaptic membrane is suggestive of liprin-alpha 2 being localized in close proximity to the postsynaptic membrane in the postsynaptic specialization. - Fig. 3: Double immunofluorescence experiments demonstrated localization of liprin-alpha 2 with the postsynaptic protein GRIP along dendrites. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Confocal microscopy images of immunocytochemistry of mouse primary cortical neurons DIV 14 labeled with antibodies against Liprin-alpha 2 and the postsynaptic protein PSD-95 demonstrate co-localization of Liprin-alpha 2 with PSD-95 (Fig. 12). @ PMID:21618221 The 254.2 anti-liprin-alpha 2 antibody was generated by immunizing mice with purified, Escherichia coli-derived GST-Liprin-α2 (amino acids 3-470) fusion protein. @ PMID:9624153 |
| SynGO annotation ID: | 4175 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |