| Annotated protein: | Liprin-alpha 4 (Protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF),-interacting protein (liprin), alpha 4). Gene symbol: PPFIA4. Taxonomy: Mus musculus (Mouse). Uniprot ID: B8QI36 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA4 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | cerebellum, glutamatergic Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Spangler SA, et al. "Differential expression of liprin-alpha family proteins in the brain suggests functional diversification" J Comp Neurol. 2011 Oct 15;519(15):3040-60 PMID:21618222 |
| Figure(s): | Fig. 8 |
| Annotation description: | Liprin-alpha 4 was shown to be enriched at parallel fiber-Purkinje cell synapses in adult mouse cerebellar cortex. Triple-labelling confocal immunofluorescence of liprin-alpha 4, calbindin (Purkinje cell dendritic trees and spines marker), and VGLUT1 (synaptic vesicle marker) . Liprin-alpha 4 did not co-distribute with calbindin but showed partial co-localization with VGLUT1 (Fig. 8). Rabbit polyclonal anti-liprin-alpha antibodies were raised against GST-tag fusion proteins containing the sequence (461-564 of liprin-a4 (EF428334)). 20/01/2021 Dnyanada: Information on antibody specificity: Figure 1K: Western blots with extracts of HeLa cells transfected with GFP-liprin alpha 4 were blotted against anti-liprinalpha4 antibody. The antibody specifically recognized a band of ~140kD representative of PPFIA4. Figure 1M: anti-liprinalpha4 antibody preadsorbed with liprin demonstrated no signal (labelled as "block"). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Immunohistochemical costaining of Liprin-alpha 4 and the presynaptic marker synapsin on sagittal adult mouse cerebellar sections showed Liprin-alpha 4 and synapsin colabeling in the molecular layer, suggesting synaptic localization in parallel fiber terminals. Confocal microscopy was used. Liprin alpha 4 antibodies were raised in rabbits against the peptide: DPLGPPHGADAEANFEC, affinity purified, and used at 1:100 (IHC). @ PMID:21618221 |
| SynGO annotation ID: | 4174 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |