Annotated protein:Liprin-alpha-2 (Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-2) (PTPRF-interacting protein alpha-2). Gene symbol: PPFIA2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BSS9
antibody wiki:
SynGO gene info:SynGO data @ PPFIA2
Ontology domain:Cellular Component
SynGO term:presynaptic cytosol (GO:0099523)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Wong MY, et al. "Liprin-alpha3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses" Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2234-2239 PMID:29439199
Figure(s):Fig. 1, Fig. S1
Annotation description:Cultured hippocampal neurons stained with antibodies against the active zone protein Bassoon, synaptic vesicle proteins - synapsin or vGlut, and liprin alpha 2 were imaged using STED microscopy. Pairwise stainings of synapses in side view were analyzed for all known active zone proteins including Liprin alpha 2 with Bassoon. To assess the localization of each protein within and outside the active zone, two masks were generated (Fig. 1A): one that outlined the nerve terminal (vGlut1 or Synapsin; Fig. 1A, blue), and one that defined the active zone (BassoonN; Fig. 1A, green). The area associated with the active zone for each protein was quantified (Fig. 1A, yellow) and compared with the area of the same protein outside the active zone (Fig. 1A, red), normalized to the sum of the area of active zone and nonactive zone com- ponents (Fig. 1E). This provided a measure of signal distribution within a nerve terminal without accounting for the signal intensity within the area. The majority of the area stained for BassoonC, RIM1, ELKS2, RIM-BP2, and Munc13-1 was at the active zone (73-90%; Fig. 1E). Liprin-α2 was widespread in the presynaptic terminal with ~50% of positively stained area outside the active zone. Unlike other known active zone proteins for which the peak was within the active zone, the peak of Liprin-αlpha 2 was away from other active-zone proteins shifted toward the synaptic vesicle cloud.

20/01/2021 Dnyanada: No information on the specificity of the liprin-alpha2 antibody used in the study.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Super resolution
Annotator(s):Arthur de Jong (ORCID:0000-0002-7620-2704)
Pascal Kaeser (ORCID:0000-0002-1558-1958)
Lab:Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
Additional literature:This study partially supports synaptic localization of liprin alpha 2 using confocal microscopy.

Figure 6: Immunohistochemical costaining of Liprin-a2 and the presynaptic marker synapsin on sagittal adult mouse cerebellar sections demonstrates co-labeling.

Figure 11: Confocal microscopy images of immunocytochemistry of primary mouse cortical neurons DIV14-21. Liprin-alpha 2 shows strong co-localization with the presynaptic protein Bassoon.

An antibody against mouse Liprin alpha 2 is raised in rabbits against the peptide: SSYHNDARSSLSAC, affinity purified, and used at 1: 50 for ICC and IHC. @ PMID:21618221

This study partially supports synaptic localization of Liprin alpha 2 using confocal microscopy.

Figure 6: Immunohistochemical costaining of Liprin-a2 and the presynaptic marker synaptophysin in the in mossy fiber endings in the stratum lucidum of the hippocampus of adult mouse brain sections demonstrates co-labeling.

Figure 9: Confocal immunofluorescence imaging of Liprin-a2 and the presynaptic marker bassoon at synapses in hippocampal neuronal cultures demonstrates co-labeling.

The rabbit polyclonal anti-liprin-alpha 2 antibody was raised against GST-tag fusion proteins containing sequences that showed the lowest amount of homology with other liprin alpha family members. 498-635 amino acids of liprin-alpha 2 (NM_003625.2). It was used at 1: 100 for ICC, 1:2000 for IHC. @ PMID:21618222
SynGO annotation ID:4172
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology