| Annotated protein: | Syntaxin-binding protein 1 (Protein unc-18 homolog 1) (Unc18-1) (Protein unc-18 homolog A) (Unc-18A). Gene symbol: STXBP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O08599 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STXBP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone cytoplasmic component (GO:0098831) |
| Synapse type(s): | cerebellum Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Siddig S, et al. "Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones" Sci Adv. 2020 Apr 15;6(16):eaay7193 PMID:32494600 |
| Figure(s): | Fig 1, 2 ,4 and 5 |
| Annotation description: | dSTORM imaging of granule cell synapses with the dendritic spines of Purkinje cells show co-localization of mGlur4 microdomains with Munc18-1 (Fig 5) and Bassoon (Fig 4). antibody KO verified: monoclonal mouse antibody against Munc18-1 (catalog no. 116011) were from Synaptic Systems (Göttingen, Germany). 28/01/2021 Dnyanada: The paper shows that mGlur4 closely associates with active zone marker Bassoon. It also shows that Munc18-1 is localised with mGlur4. SynGO ID:82 (PMID:17110441) shows that Munc18-1 plays an essential role in synaptic vesicle priming. Based on all these, the term "presynaptic active zone cytoplasmic component". |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4170 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |