| Annotated protein: | Neuromodulin (Axonal membrane protein GAP-43) (Growth-associated protein 43) (Protein F1). Gene symbol: GAP43. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P07936 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GAP43 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | thalamus |
| Annotated paper: | Feig SL, et al. "The differential distribution of the growth-associated protein-43 in first and higher order thalamic nuclei of the adult rat" Neuroscience. 2005;136(4):1147-57 PMID:16203100 |
| Figure(s): | Fig6 and 7, Table 1 |
| Annotation description: | Fig 6 shows selective presynaptic labeling of GAP-43 using DAB precipitate as a marker (indicated by the arrows) which can be observed near the plasma membrane and near synaptic vesicles. Panel A-D is rat material, E is cat. Fig 7 shows ultrastructural colocalization of GAP-43 (by DAB precipitate) and GABA (by conjugated gold) in presynaptic terminals. This colocalization is quantified in table 1. On the antibody used: "A monoclonal antiserum (clone 9-1E12, Chemicon, Temecula, CA, USA; formerly from Boehr- inger Mannheim, and Roche, Indianapolis, IN, USA) was used to identify GAP-43. Characterisation of the antiserum and preab- sorption controls have been carried out by others (Zou et al., 1996) and by the manufacturer." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Fig2: This figure shows selective presynaptic labelling of GAP-43 in the superficial dorsal horn of rats, using immuno-gold labeling in EM. The labeling looks more clear in this reference, but is only qualitative. The lead reference here has quantification. @ PMID:9303528 |
| SynGO annotation ID: | 4166 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |