| Annotated protein: | Kinesin heavy chain isoform 5C (EC 3.6.4.-) (Kinesin heavy chain neuron-specific 2) (Kinesin-1). Gene symbol: KIF5C. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P56536 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KIF5C |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | hippocampus, GABAergic spinal cord, GABAergic |
| Annotated paper: | Twelvetrees AE, et al. "Delivery of GABAARs to synapses is mediated by HAP1-KIF5 and disrupted by mutant huntingtin" Neuron. 2010 Jan 14;65(1):53-65 PMID:20152113 |
| Figure(s): | Fig 2 A-H |
| Annotation description: | "immunofluorescence and confocal microscopy with antibodies to the GABAAR g2 subunit and antibodies specific to KIF5B (Figure 2A) or KIF5C (Figure 2B) show that a proportion of KIF5 staining colocalized with GABAAR clusters in the neuronal somata and dendrites of cultured hippocampal and cortical neurons (see also Figure S2 for whole images of hippocampal neurons and for labeling of cortical neurons)." Electron microscopy revealed KIF5C-positive deposits consistently found in the vicinity of postsynaptic membranes and decorating the postsynaptic side of symmetrical type 2 contacts (Figure 2C-2H), defined as inhibitory synapses by double detection of GAD and KIF5 (Figure 2G,H). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4165 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |