| Annotated protein: | PRKCA-binding protein (Protein interacting with C kinase 1) (Protein kinase C-alpha-binding protein). Gene symbol: PICK1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9EP80 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PICK1 |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic vesicle (GO:0008021) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Haglerod C, et al. "Protein interacting with C kinase 1 (PICK1) and GluR2 are associated with presynaptic plasma membrane and vesicles in hippocampal excitatory synapses" Neuroscience. 2009 Jan 12;158(1):242-52 PMID:19071197 |
| Figure(s): | Fig1, 4 and 5 |
| Annotation description: | Fig1G-I: Double labelling for PICK1 and synaptophysin indicates that PICK1 is partly colocalized with a presynaptic marker. The inset depicts a Z-stack over a representative nerve terminal showing co-localization in three dimensions, ruling out the possibility of false co-localization due to overlapping terminals. Fig4: Electron micrographs of asymmetric synapses in CA1 stratum radiatum of hippocampus show labelling for PICK1 (small gold particles, 10 nm) where they are associated presynaptic vesicles (Fig4D inset). Fig5A: Transverse histogram shows the number of gold particles at different distances (nm) from the outer margin of the postsynaptic density specialization (PSS) (zero), in postsynaptic (negative values) and presynaptic (positive values) directions, respectively. This histogram shows that the distribution of PICK1 immunogold particles in both presynapses as well as postsynapse. Fig5C-D: The specificity of the immunogold labelling was checked by preabsorption with the immunizing peptide which nearly abolished the labelling for PICK1. Fig1B-C: siRNA sequences (E3, E5, E7) against PICK1 were used to knock down PICK1 protein in dissociated hippocampal cell cultures and these extracts were subjected to SDS-PAGE and immunostained with the PICK1 and GAPDH antibody. Comparisons were made with extracts from cells not exposed to siRNA (MOCK). The intensity of PICK1 staining was decreased after transfection for all three siRNA sequences used indicating its specificity against PICK1. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4161 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |