| Annotated protein: | Glutamate receptor ionotropic, kainate 2 (GluK2) (Glutamate receptor 6) (GluR-6) (GluR6) (Glutamate receptor beta-2) (GluR beta-2). Gene symbol: GRIK2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P39087 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIK2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | cerebellum, glutamatergic Cerebellar mossy fiber to granule cell synapse |
| Annotated paper: | Straub C, et al. "Distinct Subunit Domains Govern Synaptic Stability and Specificity of the Kainate Receptor" Cell Rep. 2016 Jul 12;16(2):531-544 PMID:27346345 |
| Figure(s): | Figure 1C, D. Figure 3C, D, E. |
| Annotation description: | Figure 3C, D, E. Using electron microscopy and antibody-mediated staining, GluK2/3 was detected at mossy fibre-granule cell (MF-GC) synapses in WT mice. The antibody used is specific, as the immunofluorescence signal disappears in slices of GluK2.A1c knock-in mice (Figure 1C, Figure 1D). Integral component of postsynaptic membrane is chosen based on current knowledge of GluK receptors being transmembrane receptors as indicated in P39087 (GRIK2_MOUSE). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Carlos Pascual-Caro (ORCID:0000-0002-2345-393X) Anjali Amrapali Vishwanath (ORCID:0000-0002-9888-0928) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) |
| Lab: | ICM - Brain and Spine Institute, Hpital Piti Salptrire, 47 Boulevard de l'Hpital, 75013 Paris, France |
| SynGO annotation ID: | 4156 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |