| Annotated protein: | RIMS-binding protein 2 (RIM-BP2). Gene symbol: RIMBP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80U40 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMBP2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of presynaptic membrane potential (GO:0099505) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Sclip A, et al. "RIM-binding proteins recruit BK-channels to presynaptic release sites adjacent to voltage-gated Ca(2+)-channels" EMBO J. 2018 Aug 15;37(16):e98637 PMID:29967030 |
| Figure(s): | Figure 7, Figure 8 |
| Annotation description: | Loss of RBP1,2 in double knockout mice show impaired presynaptic localization of BKα-channel protein (Figure 8). Presynaptic BK channels act as negative regulators of neurotransmitter release by hyperpolarizing the plasma membrane in response to AP-stimulated depolarization and Ca2+ entry through CaV channels. They show also that BK currents (amplitude and density) are abolished in the RBP1,2 double knockout mice (Figure 7). Therefore, RIM-binding proteins 1 & 2 control potassium currents and membrane potential presynaptically. 18/01/2021 Dnyanada: BK are large-conductance calcium-activated potassium channels that are enriched in the presynapse (Robitaille et al, 1993a; Knaus et al, 1996; Zhou et al, 1999; Hu et al, 2001; Ishikawa et al, 2003; Misonou et al, 2006; Nakamura & Takahashi, 2007). The current paper shows that deletion of RBP decreases and decelerates presynaptic BK currents (Figure 5, 6) by impairing presynaptic localisation of BK channels (Figure 8; as described in the annotation). Based on this, the ontology term "regulation of presynaptic membrane potential" was chosen. Note: FigS2C uses IP to demonstrate that RIMBP1,2 dKO does not alter the levels of BK channels as compared to WT. However, it is interesting to note that RIMBP1,2dKO samples stained against anti-RIMBP2 show a light band which means that RIMBP1,2 dKO model might not be completely abrogating the expression of RIMBP2. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Carlos Pascual-Caro (ORCID:0000-0002-2345-393X) Anjali Amrapali Vishwanath (ORCID:0000-0002-9888-0928) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) |
| Lab: | ICM - Brain and Spine Institute, Hpital Piti Salptrire, 47 Boulevard de l'Hpital, 75013 Paris, France |
| SynGO annotation ID: | 4141 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |