| Annotated protein: | ADP-ribosylation factor 6 (EC 3.6.5.2). Gene symbol: ARF6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62332 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ARF6 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Tagliatti E, et al. "Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse" Elife. 2016 Jan 5;5:e10116 PMID:26731518 |
| Figure(s): | Fig S1; Fig 3B |
| Annotation description: | Colocalisation of Arf6 with synaptophysin puncta in double-immunolabeling of primary hippocampal neurons is indicative of presynaptic localization. 17/02/2021 Dnyanada: Figure S1B shows that Arf6 also colocalises with presynaptic marker VGlut1. Figure S3 shows that the anti-Arf6 antibody (AVIVA SYSTEM BIOLOGY, ARP54598-P050, 1:100) used in the study did not produce a signal in arf6-knockdown condition, and the effect was rescued indicating that the antibody might be specific for Arf6. Arf6 also colocalises with postsynaptic marker "homer" and a separate annotation has been made for the term "postsynapse" (SynGO ID:4529) |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
| Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
| SynGO annotation ID: | 4129 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |