Annotated protein:ADP-ribosylation factor 6 (EC 3.6.5.2). Gene symbol: ARF6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62332
antibody wiki:
SynGO gene info:SynGO data @ ARF6
Ontology domain:Cellular Component
SynGO term:presynapse (GO:0098793)
Synapse type(s):hippocampus
Annotated paper:Tagliatti E, et al. "Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse" Elife. 2016 Jan 5;5:e10116 PMID:26731518
Figure(s):Fig S1; Fig 3B
Annotation description:Colocalisation of Arf6 with synaptophysin puncta in double-immunolabeling of primary hippocampal neurons is indicative of presynaptic localization.

17/02/2021 Dnyanada: Figure S1B shows that Arf6 also colocalises with presynaptic marker VGlut1. Figure S3 shows that the anti-Arf6 antibody (AVIVA SYSTEM BIOLOGY, ARP54598-P050, 1:100) used in the study did not produce a signal in arf6-knockdown condition, and the effect was rescued indicating that the antibody might be specific for Arf6.
Arf6 also colocalises with postsynaptic marker "homer" and a separate annotation has been made for the term "postsynapse" (SynGO ID:4529)
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):John Chua (ORCID:0000-0002-5615-1014)
Lab:Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore
SynGO annotation ID:4129
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology