Annotated protein:ADP-ribosylation factor 6 (EC 3.6.5.2). Gene symbol: ARF6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62332
antibody wiki:
SynGO gene info:SynGO data @ ARF6
Ontology domain:Cellular Component
SynGO term:presynapse (GO:0098793)
Synapse type(s):hippocampus
Annotated paper:Tagliatti E, et al. "Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse" Elife. 2016 Jan 5;5:e10116 PMID:26731518
Figure(s):S1
Annotation description:Fig S1C: Biochemical fractionation indicates enrichment of Arf6 at pH6 similarly to the SV protein synaptophysin.

Fig S1A: Endogenous Arf6 colocalizes with both presynaptic (Synaptophysin) and postsynaptic (Homer1) markers in primary rat hippocampal neurons (17 days in vitro, DIV) and triple labelling showed expression of the small GTPase at the presynaptic and postsynaptic site of single synaptic puncta.

Fig S1B: Arf6 immunoreaction product colocalises with Vglut1 and lies adjacent to homer.

18/01/2021 Dnyanada: The paper does not comment on the specificity of the antibody used.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:4128
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology