| Annotated protein: | Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1. Gene symbol: APPL1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZWA8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ APPL1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Majumdar D, et al. "An APPL1/Akt signaling complex regulates dendritic spine and synapse formation in hippocampal neurons" Mol Cell Neurosci. 2011 Mar;46(3):633-44 PMID:21236345 |
| Figure(s): | Fig1 |
| Annotation description: | Fig1A: Immunocytochemistry shows that immunoreaction product of endogenous APPL1 colocalises with presynaptic synaptic vesicle marker SV2 (marked by white arrows). Almost 90% of the SV2 clusters colocalise with APPL1 in excitatory hippocampal neurons. 18/01/2021: The paper does not comment on the specificity of the antibody used. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4123 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |