| Annotated protein: | DCC-interacting protein 13-alpha (Dip13-alpha) (Adapter protein containing PH domain, PTB domain and leucine zipper motif 1). Gene symbol: APPL1. Taxonomy: Homo sapiens (Human). Uniprot ID: Q9UKG1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ APPL1 |
| Ontology domain: | Biological Process |
| SynGO term: | maintenance of synapse structure (GO:0099558) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Majumdar D, et al. "An APPL1/Akt signaling complex regulates dendritic spine and synapse formation in hippocampal neurons" Mol Cell Neurosci. 2011 Mar;46(3):633-44 PMID:21236345 |
| Figure(s): | Fig1 and 2 |
| Annotation description: | Fig1B: The APPL1 siRNA knocked down the endogenous expression of APPL1 by approximately 72% compared with either pSUPER empty vector or a scrambled siRNA. Fig1C-F: Transfection of APPL1 siRNA into neurons caused a significant decrease in the spine density as determined by GFP fluorescence as well as phalloidin staining compared to pSUPER empty vector or a scrambled siRNA. Expression of human GFP-APPL1 with the APPL1 siRNA completely rescued the siRNA-promoted defect in the development of spines and synapses indicating the APPL1 siRNA-mediated defect observed in the spine and synapse formation was due to endogenous loss of the protein. Fig2A-D: Overexpression of GFP-APPL1 lead to a 1.6 fold increase in the number of spines as compared to control. Similarly, expression of GFP-APPL1 showed a significant increase in the number of SV2 and PSD-95 clusters compared with GFP expressing controls. Dnyanada: The species of the protein used is homo sapien because the overexpressed APPL1 was derived from human cDNA. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4122 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |