| Annotated protein: | Sorting nexin-27. Gene symbol: SNX27. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3UHD6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNX27 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Cai L, et al. "Deficiency of sorting nexin 27 (SNX27) leads to growth retardation and elevated levels of N-methyl-D-aspartate receptor 2C (NR2C" Mol Cell Biol. 2011 Apr;31(8):1734-47 PMID:21300787 |
| Figure(s): | Fig 7 |
| Annotation description: | Fig 7A: Immunoprecipitation studies indicate that NR2C coprecipitates with SNX27. Fig 7B: Immunocytochemistry in primary cerebellar neurons shows that SNX27 colocalises with NR2C. Fig 8A: Immunoblot analysis shows that the level of NR2C was increased by about 35 to 40% in the SNX27-/- brain compared to the wild-type brain. Fig 8B: Real-time PCR demonstrated that the mRNA levels of NR2C transcripts in brain tissues from wild-type and knockout mice were not significantly different. Fig 8D: Primary cortical neurons from SNX27+/+ and SNX27-/- mice were transfected with Myc-NR2C and pulse-chased with the anti-Myc antibody for various periods. 85% more NR2C was endocytosed in SNX27+/+ than in SNX27-/- neurons as evidenced by the greater number of internalized NR2C (red puncta) in SNX27+/+ neurons at 5 and 15 min after pulse-chase than in wild-type neurons. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4114 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |