| Annotated protein: | Protein VAC14 homolog. Gene symbol: VAC14. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80WQ2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VAC14 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic endosome (GO:0098830) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Zhang Y, et al. "Modulation of synaptic function by VAC14, a protein that regulates the phosphoinositides PI(3,5)P(2) and PI(5)P" EMBO J. 2012 Aug 15;31(16):3442-56 PMID:22842785 |
| Figure(s): | Fig2 |
| Annotation description: | Fig 2B: VAC14 puncta partially colocalized with both EEA1 (23±12%) and LAMP1 (29±9%) in neurites. Fig 2C: VAC14 also colocalises with presynaptic terminal markers synapsin, synaptotagmin and VAMP/synaptobrevin. Fig S7D-E: The specificity of VAC14 is demonstrated by the lack of staining in VAC14 -/- KO neurons. Figure S1A: Specificity of anti-VAC14 antibody was verified by the absence of a band in VAC14 KO brain lysates. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4105 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |