| Annotated protein: | Ezrin (Cytovillin) (Villin-2) (p81). Gene symbol: EZR. Taxonomy: Mus musculus (Mouse). Uniprot ID: P26040 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EZR |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Merino P, et al. "Urokinase-type plasminogen activator (uPA) promotes ezrin-mediated reorganization of the synaptic cytoskeleton in the ischemic brain" J Biol Chem. 2018 Jun 15;293(24):9234-9247 PMID:29720403 |
| Figure(s): | Fig 5 |
| Annotation description: | Fig5: phalloidin/rhodamine-coupled actin assay was used to detect new actin polymerization in the postsynaptic terminal. To investigate whether actin polymerization is mediated by ezrin, neurons treated with Urokinase-type plasminogen activator (uPA) were tested for new actin structures in the presence of ezrin siRNA. The area of newly formed actin in the presence of uPA shifted from 84.9 ± 1.51 μm2 to 38.8 ± 4.24 μm2 indicating that ezrin down-regulation abrogates the effect of uPA on actin formation. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4103 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |