| Annotated protein: | Intercellular adhesion molecule 5 (ICAM-5) (Telencephalin). Gene symbol: ICAM5. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q60625 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ICAM5 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse assembly (GO:0051963) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ning L, et al. "Interactions between ICAM-5 and beta1 integrins regulate neuronal synapse formation" J Cell Sci. 2013 Jan 1;126(Pt 1):77-89 PMID:23015592 |
| Figure(s): | Fig 1 |
| Annotation description: | In order to test the function of ICAM5 in synapse assembly; synapsin I and PSD-95 were used as markers for pre- and post-synapses respectively, and the colocalization of the two markers were used to evaluate the level of the pre- and post-synaptic contacts. Fig 1A-B: At 15 day in vitro (DIV), in ICAM-5-/- neurons, synapsin I puncta accumulated on the spine heads while in WT neurons, they scatter along the dendritic shafts. The overlap between synapsin I and PSD-95 was significantly increased in ICAM-5-/- (78%±3%) neurons compared with WT (51%±5%) and the colocalization mainly increased in spine heads. To study whether the increased synaptic contacts in ICAM-5-/- neurons are functionally active, the inter-event intervals and amplitude of miniature excitatory postsynaptic currents (mEPSCs) in cultured WT and ICAM-5-deficient neurons were recorded. Fig 1C-E: Compared with WT, ICAM-5-/- neurons exhibited a significant increase in the frequency (decrease in the inter-event intervals) of mEPSCs, however, there was no significant effect on the amplitude. The mean relative inter-event interval was 53±11% shorter in ICAM-5-/- than in WT neurons implying a significant increase in the frequency of mEPSC. 18/01/2021: The term "regulation of synapse assembly" was chosen instead of "regulation of synapse maturation" as the paper demonstrates the role of ICAM5 in fully mature synapse. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Using cultured hippocampal neurons, the paper examines the function of ICAM5 (TLCN) in spine development and synaptogenesis. Overexpression of ICAM5 causes an increase in the density of dendritic filopodia and a concomitant decrease in the density of spines (Fig 5 &6); TLCN-deficient mice showed a decreased density of filopodia and an acceleration of spine maturation in vitro as well as in vivo (Fig 8). @ PMID:16467526 |
| SynGO annotation ID: | 4099 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |