| Annotated protein: | Neurexin-2 (Neurexin II-alpha) (Neurexin-2-alpha). Gene symbol: NRXN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: E9Q7X7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRXN2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor activity (GO:0098962) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Born G, et al. "Genetic targeting of NRXN2 in mice unveils role in excitatory cortical synapse function and social behaviors" Front Synaptic Neurosci. 2015 Feb 19;7:3 PMID:25745399 |
| Figure(s): | Fig 4 and 5 |
| Annotation description: | Fig4C-F: Miniature excitatory postsynaptic currents (mEPSC) in presence of 1 μM TTX to abolish action potential driven network activity and 10 μM bicuculline to pharmacologically isolate excitatory (glutamatergic) events showed a 65% reduction in Nrxn2a KO mice compared to WT. The amplitude and decay times of mEPSC were unchanged indicating that the KO perhaps does not grossly affect transmitter quanta and receptors properties. Fig 4G-H: In order to check if the reduced mEPSC frequency was due to altered synapse density in brain tissue, quantitative electron microscopy was performed. The number of excitatory synapses, the area density of synaptic vesicles, length of synaptic zones, the width of synaptic clefts and the area of synaptic terminals were not altered between Nrxn2A KO and WT suggesting that the differences observed in spontaneous excitatory transmission are not caused by structural defects. Fig 5A-D: To evaluate if the reduction in mEPSC frequency is caused due to presynaptic mechanisms, paired-pulse facilitation paradigm was conducted. PPF was significantly reduced at most intervals in Nrxn2A-KO neurons. Fig 5G-H: Quantitative analysis of NMDA/AMPA ratios revealed an almost 25% reduction in Nrxn2α KO neurons as compared to WT. 18/01/2021 Dnyanada: Based on the reduction in NMDAR-dependent decay time and NMDAR-mediated responses in Nrxn2α KO excitatory synapses, the term "regulation of postsynaptic neurotransmitter receptor activity" was used. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Whole-cell patch clamp |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4092 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |