| Annotated protein: | Sorting nexin-6 [Cleaved into: Sorting nexin-6, N-terminally processed]. Gene symbol: SNX6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6P8X1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNX6 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Niu Y, et al. "Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory" Elife. 2017 Jan 30;6:e20991 PMID:28134614 |
| Figure(s): | Fig 9 |
| Annotation description: | Fig 9A: Snx6 gene was eliminated in a small subset of hippocampal neurons by injection of organotypic hippocampal slice culture from Snx6fl/fl mouse with recombinant adeno-associated virus (AAV) coexpressing EGFP and the Cre recombinase. Fig 9B-D: Results from simultaneous recording the evoked EPSCs (eEPSCs) on infected and adjacent uninfected CA1 pyramidal neurons showed that AMPAR-mediated eEPSCs were significantly impaired by about 50% with ablation of SNX6 whereas NMDAR-mediated eEPSCs and the pair-pulse ratio of AMPAR eEPSCs was unaffected indicating that the impairment of AMPAR eEPSCs is due to decrease in the number of AMPARs in the postsynaptic membrane but not the reduction of presynaptic glutamate release. Fig 9G-J: A decrease in the surface expression of GluA1 and GluA2 was observed in Nestin Cre SNX6 fl/fl (KO) neurons as compared to SNX6 fl/fl (control). This effect was fully rescued by overexpressing SNX6. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Electrophysiology (generic) |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4091 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |