| Annotated protein: | Sorting nexin-27. Gene symbol: SNX27. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3UHD6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNX27 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Wang X, et al. "Loss of sorting nexin 27 contributes to excitatory synaptic dysfunction by modulating glutamate receptor recycling in Down's syndrome" Nat Med. 2013 Apr;19(4):473-80 PMID:23524343 |
| Figure(s): | Fig 2, 3, 4 and supplementary Fig 7 |
| Annotation description: | Fig2h: Miniature excitatory postsynaptic currents (mEPSCs) from CA1 hippocampal neurons displayed a significantly lower amplitude in Snx27+/- neurons compared to Snx27+/+ neurons. Fig2i: LTP induced by high-frequency trains (100 Hz for 1 s) was significantly reduced in Snx27+/- mice compared to controls. Both these results suggest that Snx27+/-might affect both AMPAR- and NMDAR-dependent synaptic events. Fig 3a: Western blot analysis shows that Snx27+/- mice had lower amounts of GluR1, GluR2, NR1, NR2A and NR2B in both synaptosomal membranes and PSD fractions compared to Snx27+/+ littermates. Fig 4d-f: Cell surface biotinylation experiments show that overexpression of SNX27 resulted in increased cell surface amounts of GluR1 and NR1 in GluR1-HEK293 and NR1-NR2A-HEK293 cells. Conversely, knockdown of SNX27 resulted in reduced cell surface expression of GluR1 and NR1. This effect was also evident in primary neurons; with a decrease in cell surface expression of GluR1 and NR1 in primary neurons derived from Snx27+/- mice. Fig 4g-h: Receptor recycling assay shows that overexpression of SNX27 decreased the amount of internalized biotinylated GluR1 in GluR1-HEK293 cells. Consistent with these overexpression experiments, biotin-labelled GluR1 after treatment with SNX27 siRNA showed increased internalized biotinylated GluR1as compared to control siRNA. Supplementary Fig 7: SNX27 does not influence endocytosis of GluR1. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Field recordings Western blot |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4090 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |