| Annotated protein: | Vacuolar protein sorting-associated protein 35 (Maternal-embryonic 3) (Vesicle protein sorting 35). Gene symbol: VPS35. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Biological Process |
| SynGO term: | modulation of chemical synaptic transmission (GO:0050804) |
| Synapse type(s): | basal ganglia, dopaminergic |
| Annotated paper: | Cataldi S, et al. "Altered dopamine release and monoamine transporters in Vps35 p.D620N knock-in mice" NPJ Parkinsons Dis. 2018 Aug 21;4:27 PMID:30155515 |
| Figure(s): | Fig 4, S7 |
| Annotation description: | Fig a-c: Fast scan cyclic voltammetry indicates elevated dopamine release in VKI slices (VPS35 KI slices) compared to WT. Fig 4f: Bathing slices with Quinpirole (a D2 agonist), shows enhanced inhibition of dopamine release in VKI slices when compared to WT. Fig4d: Decay times for the evoked dopamine transients is slower in VKI slices than WT, suggesting slower re-uptake of extracellular dopamine. Fig6: Western Blot analysis from striatum shows reduced total DAT protein levels and higher VMAT2 levels in VKI mice compared to WT. The enhanced dopamine evoked response can be correlated to elevated VMAT2 levels; while the increased decay time of dopamine release can be correlated to reduced DAT protein levels. Based on the results above, one might infer deficits in activity-dependent transporter recycling (DAT) to the plasma membrane via VPS35-positive endosomes in VKI mice. 18/01/2021 Dnyanada: The general term "modulation of chemical synaptic transmission" was used due to limited synaptic evidence. However, it is an interesting paper that demonstrates the functional role of VPS35 in dopaminergic neurons. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) Western blot |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4089 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |