| Annotated protein: | Somatostatin [Cleaved into: Antrin; Somatostatin-28; Somatostatin-14; Neuronostatin (NST)]. Gene symbol: SST. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60041 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SST |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Kim S, et al. "Loss of IQSEC3 Disrupts GABAergic Synapse Maintenance and Decreases Somatostatin Expression in the Hippocampus" Cell Rep. 2020 Feb 11;30(6):1995-2005.e5 PMID:32049026 |
| Figure(s): | Figure 4 and S6 |
| Annotation description: | Figure 4G: Representative eIPSC traces recorded from DG granule neurons in acute DG slices from Sst-IRES-Cre mice. Figure 4H: Summary of fitted linear input/output slopes from Figure 4G. Evoked IPSC was significantly reduced in IQSEC3 KD mice, this effect was rescued by addition of DIO-SST. Figure S6A: Representative images of the cultured hippocampal neurons treated with DMSO, SST, SST+cSST [cyclosomatostatin], and SST+SecinH3 analyzed by staining surface-exposed GABAARγ2 (red) and MAP2 (green). Figure S6B: Summary graphs of the effects of SST peptides on surface GABAARγ2 puncta density. Bathing hippocampal neurons with SST increases the surface GABAARγ2 puncta. This effect was completely abrogated by cotreatment with SST antagonist, cyclosomatostatin or inhibitor of the cytohesin ARF-GEF family, SecinH3. Overall, SST indicates to regulate the GABARγ2 levels in the synapse. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Whole-cell patch clamp |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4076 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |