| Annotated protein: | Calcium-dependent secretion activator (Uncoordinated protein 31). Gene symbol: UNC-31. Taxonomy: Caenorhabditis elegans (Worm). Uniprot ID: Q23658 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CADPS SynGO data @ CADPS2 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic dense core vesicle exocytosis (GO:0099525) |
| Annotated paper: | Lin XG, et al. "UNC-31/CAPS docks and primes dense core vesicles in C. elegans neurons" Biochem Biophys Res Commun. 2010 Jul 2;397(3):526-31 PMID:20515653 |
| Figure(s): | Figure 1 |
| Annotation description: | The paper uses high-resolution membrane capacitance (Cm) measurement combined with Ca2+-uncaging stimuli to monitor the effect of Unc31 KO in DCV exocytosis (as described in PMID:18031683). Figure 1A: Under Step-like elevations in intracellular calcium, membrane capacitance was significantly reduced in Unc31 mutant (unc-31e928) relative to WT indicating that Unc31 mutants defect exocytosis in ALA neurons of C. elegans. The exocytotic defect was fully rescued by reintroduction of full-length unc-31 cDNA. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Whole-cell patch clamp |
| Annotator(s): | Dnyanada Sahasrabudhe (ORCID:0000-0003-2916-7616) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | This paper introduces a new technique to measure DCV exocytosis kinetics using high-resolution membrane capacitance (Cm) combined with Ca2+ uncaging stimuli. Figure 2A: Average Cm responses (bottom) induced by similar [Ca2+]i elevations (top) in wild-type (black trace), unc-31 mutant (red trace), unc-64 mutant (blue trace), and unc-18 mutant (grey trace) cells are compared. The inset compares the amplitude of burst exocytosis between wild-type, unc-31, unc-64, unc-18, and tag-81 mutant cells. The exocytotic burst is quantified as the amplitude of the exponential fit to the Cm trace. Unc18 is well known to play a role in DCV priming and exocytosis. Drastic reduction (~85%) in the amplitude of burst exocytosis of the Unc18 mutant validates the method and was used as a positive control. Reduction in the amplitude of burst exocytosis for Unc31 mutant indicates its role in DCV exocytosis. @ PMID:18031683 |
| SynGO annotation ID: | 4065 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |